Please use this identifier to cite or link to this item: https://doi.org/10.1038/nature09531
Title: A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity
Authors: Chia, N.-Y.
Chan, Y.-S.
Feng, B.
Lu, X.
Orlov, Y.L.
Moreau, D.
Kumar, P.
Yang, L.
Jiang, J.
Lau, M.-S.
Huss, M.
Soh, B.-S.
Kraus, P.
Li, P.
Lufkin, T.
Lim, B.
Clarke, N.D.
Bard, F.
Ng, H.-H. 
Issue Date: 11-Nov-2010
Citation: Chia, N.-Y., Chan, Y.-S., Feng, B., Lu, X., Orlov, Y.L., Moreau, D., Kumar, P., Yang, L., Jiang, J., Lau, M.-S., Huss, M., Soh, B.-S., Kraus, P., Li, P., Lufkin, T., Lim, B., Clarke, N.D., Bard, F., Ng, H.-H. (2010-11-11). A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. Nature 468 (7321) : 316-320. ScholarBank@NUS Repository. https://doi.org/10.1038/nature09531
Abstract: The derivation of human ES cells (hESCs) from human blastocysts represents one of the milestones in stem cell biology. The full potential of hESCs in research and clinical applications requires a detailed understanding of the genetic network that governs the unique properties of hESCs. Here, we report a genome-wide RNA interference screen to identify genes which regulate self-renewal and pluripotency properties in hESCs. Interestingly, functionally distinct complexes involved in transcriptional regulation and chromatin remodelling are among the factors identified in the screen. To understand the roles of these potential regulators of hESCs, we studied transcription factor PRDM14 to gain new insights into its functional roles in the regulation of pluripotency. We showed that PRDM14 regulates directly the expression of key pluripotency gene POU5F1 through its proximal enhancer. Genome-wide location profiling experiments revealed that PRDM14 colocalized extensively with other key transcription factors such as OCT4, NANOG and SOX2, indicating that PRDM14 is integrated into the core transcriptional regulatory network. More importantly, in a gain-of-function assay, we showed that PRDM14 is able to enhance the efficiency of reprogramming of human fibroblasts in conjunction with OCT4, SOX2 and KLF4. Altogether, our study uncovers a wealth of novel hESC regulators wherein PRDM14 exemplifies a key transcription factor required for the maintenance of hESC identity and the reacquisition of pluripotency in human somatic cells. © 2010 Macmillan Publishers Limited. All rights reserved.
Source Title: Nature
URI: http://scholarbank.nus.edu.sg/handle/10635/99836
ISSN: 00280836
DOI: 10.1038/nature09531
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

274
checked on Jul 14, 2018

WEB OF SCIENCETM
Citations

259
checked on Jun 18, 2018

Page view(s)

42
checked on Jun 29, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.