Clonal propagation of guava (Psidium guajava L.) from seedlings and grafted plants and adventitious shoot formation in vitro

Abstract

Different parts of guava (Psidium guajava L.) seedlings and the nodal segments of grafted plants were used as explants for in vitro culture. A high frequency (75-100%) of shoot regeneration was obtained from seedling hypocotyl, shoot tip and nodal segments cultured in MS medium with or without BA. The optimal BA Level for shoot tips and nodal segments is 0.1 mg l-1. Nodal segments form regenerated shoots were used for further multiplication; an average of 3.2 shoots segment-1 could be obtained after a period of 8 weeks in medium with 0.5 mg l-1 BA. Shoots were also regenerated from leaf explants. Regenerated shoots were rooted in basal medium with 100% rooting frequency and more than 90% of the plantlets were successfully established in soil. © 1989.