Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/99069
Title: KINETIC IS AN EFFECTOR FOR ENDOPLASMIC RETICULUM MEDIATED CELL PROTRUSION SELECTION AND FOCAL ADHESION FORMATION FOR PROTRUSION STABILIZATION DURING CHEMOTAXIS
Authors: NG INN CHUAN
Keywords: Kinectin, epithelial-to-mesenchymal transition, chemotaxis, endoplasmic reticulum, protrusion stabilization, focal adhesion
Issue Date: 16-Jan-2013
Source: NG INN CHUAN (2013-01-16). KINETIC IS AN EFFECTOR FOR ENDOPLASMIC RETICULUM MEDIATED CELL PROTRUSION SELECTION AND FOCAL ADHESION FORMATION FOR PROTRUSION STABILIZATION DURING CHEMOTAXIS. ScholarBank@NUS Repository.
Abstract: Kinectin mediates ER transport to promote maturation of focal complexes (FXs) to focal adhesions (FAs). Knockdown of kinectin in HeLa cells caused morphological changes reminiscent of mesenchymal-to-epithelial transition and reduced migration. Here, I investigated the roles of kinectin in epithelial-to-mesenchymal transition (EMT) and cell migration. I determined that kinectin is not a mesenchymal marker but contributes to the acquisition of mesenchymal phenotype. The expression of kinectin was not upregulated after TGF-?1-induced EMT. However, kinectin supported morphological changes and cell migration during culture density-induced EMT. I also demonstrated that kinectin contributes to the directional bias but not motility of migration. Kinectin-dependent ER transport promotes preferential maintenance of leading protrusions oriented towards the chemoattractant source. Kinectin mediated ER transport to correctly oriented protrusions while ER promoted the formation of FAs for protrusion stabilization.
URI: http://scholarbank.nus.edu.sg/handle/10635/99069
Appears in Collections:Ph.D Theses (Open)

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