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|Title:||Single-cell electroporation using proton beam fabricated biochips|
Proton beam writing
Single cell electroporation
|Citation:||Homhuan, S., Zhang, B., Sheu, F.-S., Bettiol, A.A., Watt, F. (2010). Single-cell electroporation using proton beam fabricated biochips. Proceedings of SPIE - The International Society for Optical Engineering 7716 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.850657|
|Abstract:||We report the design and fabrication of a novel single cell electroporation biochip fabricated by the Proton Beam Writing technique (PBW), a new technique capable of direct-writing high-aspect-ratio nano and microstructures. The biochip features nickel micro-electrodes with straight-side walls between which individual cells are positioned. By applying electrical impulses across the electrodes, SYTOX® Green nucleic acid stain is incorporated into mouse neuroblastoma (N2a) cells. When the stain binds with DNA inside the cell nucleus, green fluorescence is observed upon excitation from a halogen lamp. Three parameters; electric field strength, pulse duration, and the number of pulses have been considered and optimized for the single cell electroporation. The results show that our biochip gives successfully electroporated cells. This single cell electroporation system represents a promising method for investigating the introduction of a wide variety of fluorophores, nanoparticles, quantum dots, DNAs and proteins into cells. © 2010 SPIE.|
|Source Title:||Proceedings of SPIE - The International Society for Optical Engineering|
|Appears in Collections:||Staff Publications|
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