Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gkr1247
Title: Gene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility
Authors: Lim, C.J.
Whang, Y.R.
Kenney, L.J.
Yan, J. 
Issue Date: Apr-2012
Citation: Lim, C.J., Whang, Y.R., Kenney, L.J., Yan, J. (2012-04). Gene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility. Nucleic Acids Research 40 (8) : 3316-3328. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkr1247
Abstract: Nucleoid-associated proteins are bacterial proteins that are responsible for chromosomal DNA compaction and global gene regulation. One such protein is Escherichia coli Histone-like nucleoid structuring protein (H-NS) which functions as a global gene silencer. Whereas the DNA-binding mechanism of H-NS is well-characterized, its paralogue, StpA which is also able to silence genes is less understood. Here we show that StpA is similar to H-NS in that it is able to form a rigid filament along DNA. In contrast to H-NS, the StpA filament interacts with a naked DNA segment to cause DNA bridging which results in simultaneous stiffening and bridging of DNA. DNA accessibility is effectively blocked after the formation of StpA filament on DNA, suggesting rigid filament formation is the important step in promoting gene silencing. We also show that >1mM magnesium promotes higher order DNA condensation, suggesting StpA may also play a role in chromosomal DNA packaging. © 2011 The Author(s).
Source Title: Nucleic Acids Research
URI: http://scholarbank.nus.edu.sg/handle/10635/96698
ISSN: 03051048
DOI: 10.1093/nar/gkr1247
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

39
checked on Sep 19, 2018

WEB OF SCIENCETM
Citations

38
checked on Sep 4, 2018

Page view(s)

22
checked on Sep 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.