Please use this identifier to cite or link to this item: https://doi.org/10.1088/1742-6596/28/1/017
Title: Determination of agarose gel pore size: Absorbance measurements vis a vis other techniques
Authors: Narayanan, J. 
Xiong, J.-Y. 
Liu, X-Y. 
Issue Date: 1-Jan-2006
Citation: Narayanan, J., Xiong, J.-Y., Liu, X-Y. (2006-01-01). Determination of agarose gel pore size: Absorbance measurements vis a vis other techniques. Journal of Physics: Conference Series 28 (1) : 83-86. ScholarBank@NUS Repository. https://doi.org/10.1088/1742-6596/28/1/017
Abstract: The absorbance measurements in the wavelength range 700 nm to 800 nm were used to probe the agarose gel topology evolution and extract the pore size of the trapped solvent. By following the changes in absorbance and pore size, the gelation process could be clearly divided into three stages - induction stage, gelation stage and pseudo-equilibrium stage. The gelation mechanism is explained as a nucleation and growth process. Following the kinetics of gelation using dynamic light scattering is complicated by multiple scattering (for high concentrations) and large fluctuations in intensity and relaxation time. Comparatively, scanning the absorption spectrum is fast and the method is suitable for a wide range of concentrations and setting temperatures. Pore size determination using absorbance is a fast and non-invasive method when compared to the DNA electrophoresis measurements, which extend over several hours and use probe diffusion. © 2006 IOP Publishing Ltd.
Source Title: Journal of Physics: Conference Series
URI: http://scholarbank.nus.edu.sg/handle/10635/96191
ISSN: 17426588
DOI: 10.1088/1742-6596/28/1/017
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