Please use this identifier to cite or link to this item: https://doi.org/10.1071/CH09112
Title: Immobilized whole cells as effective catalysts for chiral alcohol production
Authors: Ng, J.F.
Jaenicke, S. 
Issue Date: 2009
Source: Ng, J.F., Jaenicke, S. (2009). Immobilized whole cells as effective catalysts for chiral alcohol production. Australian Journal of Chemistry 62 (9) : 1034-1039. ScholarBank@NUS Repository. https://doi.org/10.1071/CH09112
Abstract: Recombinant Escherichia coli overexpressing the gene LbADH, which encodes for an alcohol dehydrogenase from Lactobacillus brevis, was successfully transformed and cultured. The cells are able to catalyze the reduction of pro-chiral ketones, e.g. ethyl acetoacetate into R-()ethyl hydroxybutyrate (EHB) with high conversion and enantiomeric excess 99%. Immobilizing the whole cells in alginate beads leads to a catalyst with improved stability and ease of handling while maintaining the high activity of the free cells. The whole-cell catalyst was tested in a stirred batch reactor (CSTR) and in a continuously operated packed-bed reactor. An Mg2+ concentration of 2 mM was crucial for maintaining the activity of the biocatalyst. After a partial optimization of the process conditions, a productivity of 1.4 gEHB gwcw -1 h-1 could be maintained in a continuous flow reactor over a prolonged period of time. © CSIRO 2009.
Source Title: Australian Journal of Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/95469
ISSN: 00049425
DOI: 10.1071/CH09112
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

9
checked on Feb 13, 2018

WEB OF SCIENCETM
Citations

8
checked on Nov 20, 2017

Page view(s)

28
checked on Feb 17, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.