Please use this identifier to cite or link to this item: https://doi.org/10.1039/c0an00838a
Title: Visual SNP genotyping using asymmetric PCR and split DNA enzymes
Authors: Neo, J.L.
Aw, K.D.
Uttamchandani, M. 
Issue Date: 21-Apr-2011
Citation: Neo, J.L., Aw, K.D., Uttamchandani, M. (2011-04-21). Visual SNP genotyping using asymmetric PCR and split DNA enzymes. Analyst 136 (8) : 1569-1572. ScholarBank@NUS Repository. https://doi.org/10.1039/c0an00838a
Abstract: Harnessing and applying genomic technologies in resource limited environments demand a next generation of platforms, which are convenient, quick and easy to apply. We describe here a visual colour change assay that can be applied to SNP genotyping, which unlike traditional methods, does not adopt complicated procedures or expensive instrumentation, desirable features in bringing genetic capabilities outside the laboratory. Our strategy involved a two-step method that first enriched target genomic regions using asymmetric PCR, followed by direct in situ application of split DNA probes. In the presence of target sequences that perfectly matched the complementary probes, the split probes reassembled active DNAzymes, which catalysed a colour change reaction. A single-base mismatch (indicative of a polymorphism) prevented this reassembly and colour change, providing the means for accurate SNP calling. This is the first report, to our knowledge, that demonstrates successful visual SNP genotyping of actual human DNA samples using DNAzymes. © 2011 The Royal Society of Chemistry.
Source Title: Analyst
URI: http://scholarbank.nus.edu.sg/handle/10635/95407
ISSN: 00032654
DOI: 10.1039/c0an00838a
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

16
checked on Aug 16, 2018

WEB OF SCIENCETM
Citations

17
checked on Aug 1, 2018

Page view(s)

41
checked on Aug 17, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.