Please use this identifier to cite or link to this item: https://doi.org/10.1002/elps.200500233
Title: Separation and determination of toxic pyrrolizidine alkaloids in traditional Chinese herbal medicines by micellar electrokinetic chromatography with organic modifier
Authors: Yu, L. 
Xu, Y.
Feng, H.
Li, S.F.Y. 
Keywords: Chinese herbal medicine
Kuan donghua
Micellar electrokinetic chromatography
Pyrrolizidine alkaloids
Qian liguang
Issue Date: Sep-2005
Citation: Yu, L., Xu, Y., Feng, H., Li, S.F.Y. (2005-09). Separation and determination of toxic pyrrolizidine alkaloids in traditional Chinese herbal medicines by micellar electrokinetic chromatography with organic modifier. Electrophoresis 26 (17) : 3397-3404. ScholarBank@NUS Repository. https://doi.org/10.1002/elps.200500233
Abstract: A simple and rapid micellar electrokinetic chromatography (MEKC) method was developed for the separation and determination of four toxic pyrrolizidine alkaloids (PAs) (senkirkine, senecionine, retrorsine, and seneciphylline) in two traditional Chinese herbal medicines (Qian liguang and Kuan donghua). Separation was performed in the running buffer consisting of 20 mm borate, 30 mM SDS, and 20% methanol at pH 9.1. With the optimized separation conditions, four PAs were separated in 17 min by a single run. The calibration curves showed good linearity with correlation efficiencies (R2) between 0.9940 and 0.9988. RSDs in migration time and peak area were 0.31, 0.40, 0.39, 0.48% and 3.28, 3.48, 4.16, 3.42% for senkirkine, senecionine, retrorsine, and seneciphylline, respectively. Limits of detection (S/N = 3) varied from 1.19 to 2.70 μg/mL. The proposed method was applied to determine the PAs extracted from Chinese herbal medicines (Qian liguang and Kuan donghua). PA of senkirkine in Kuan donghua was detected and the amount was found to be 79.1 μg/g. The results obtained indicate that the proposed MEKC method could potentially become an effective alternative tool for qualification control and quantitative analysis of herbal medicines in pharmaceutical industry. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Electrophoresis
URI: http://scholarbank.nus.edu.sg/handle/10635/94793
ISSN: 01730835
DOI: 10.1002/elps.200500233
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