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Title: Osmium carbonyl clusters containing labile ligands hyperstabilize microtubules
Authors: Kien, V.K.
Weng, K.L. 
Lim, L.H.K.
Issue Date: 15-Jun-2009
Source: Kien, V.K., Weng, K.L., Lim, L.H.K. (2009-06-15). Osmium carbonyl clusters containing labile ligands hyperstabilize microtubules. Chemical Research in Toxicology 22 (6) : 1116-1122. ScholarBank@NUS Repository.
Abstract: A study into the possible molecular targets of the osmium carbonyl cluster Os3(CO) 10(NCCH 3) 2 (2) in the ER- breast carcinoma (MDA-MB-231) cell line was carried out. Infrared and 1H NMR analyses of cells treated with 2 showed the formation of carboxylato- and thiolato-bridged clusters from the interaction with intracellular carboxylic acid and sulfhydryl residues. The cytotoxicity of 2 was reduced in the presence of fetal bovine serum, and measurement with Ellman's reagent as well as fluorescence confocal microscopy with tetramethylrhodamine-5-maleimide staining all demonstrated binding to intracellular sulfhydryl groups leading up to cell disruption. Tubulin-FITC antibody staining of treated cells showed disruption of the microtubules, and a tubulin polmerization assay showed that 2 induced hyperstabilization of the microtubules. © 2009 American Chemical Society.
Source Title: Chemical Research in Toxicology
ISSN: 0893228X
DOI: 10.1021/tx900056a
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