Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2011.04.073
Title: Hydrazone-based ligands for micro-solid phase extraction-high performance liquid chromatographic determination of biogenic amines in orange juice
Authors: Basheer, C.
Wong, W.
Makahleh, A.
Tameem, A.A.
Salhin, A.
Saad, B.
Lee, H.K. 
Keywords: Biogenic amines
High performance liquid chromatography
Hydrazone
Micro-solid phase extraction
Sol-gel
Issue Date: 15-Jul-2011
Citation: Basheer, C., Wong, W., Makahleh, A., Tameem, A.A., Salhin, A., Saad, B., Lee, H.K. (2011-07-15). Hydrazone-based ligands for micro-solid phase extraction-high performance liquid chromatographic determination of biogenic amines in orange juice. Journal of Chromatography A 1218 (28) : 4332-4339. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2011.04.073
Abstract: Eight hydrazone-based ligands were synthesized, trapped in a silica sol-gel matrix, and were subsequently used in the micro-solid phase extraction (μ-SPE) of biogenic amines (BAs). The BAs investigated were tryptamine, phenylethylamine, putrescine, histamine, tyramine and spermidine. Prior to the extraction, dansyl chloride was added to the samples which were heated to 70°C for 10min. The samples were extracted with μ-SPE, after which analytes were desorbed using acetonitrile via ultrasonication. The extracts were analysed by high performance liquid chromatography (HPLC) with ultraviolet detection. Of the eight ligands investigated as sorbents, benzophenone 2,4-dinitrophenylhydrazone was found to be the most promising. The enhanced π-π interaction between the analytes and the ligand facilitated the adsorption process. Under the most suitable extraction conditions, the method demonstrated good linearity with correlation coefficient of more than 0.985 over a concentration range of 1-50μgL-1. Satisfactory repeatability with relative standard deviations of 7.43-11.30% (n=3) were obtained. Detection limits ranged from 3.8 to 31.3ngL-1. The μ-SPE method exhibited lower recoveries (71.5-87.4%) when compared to the solid phase extraction technique (79.7-95.0%), but enrichment factors of 94-460 were obtained. The proposed μ-SPE-HPLC method was applied to the determination of BAs in orange juice purchased from local supermarkets, with satisfactory results. The orange juices were characterized by the presence of relatively high levels of putrescine (range, 550-2210μgL-1) but tryptamine and phenylethylamine were not detected in any of the tested samples. © 2011 Elsevier B.V.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/93975
ISSN: 00219673
DOI: 10.1016/j.chroma.2011.04.073
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

19
checked on Jul 18, 2018

WEB OF SCIENCETM
Citations

15
checked on Jun 4, 2018

Page view(s)

38
checked on May 11, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.