Please use this identifier to cite or link to this item: https://doi.org/10.5740/jaoacint.13-175
Title: Determination of total antioxidant capacity by oxygen radical absorbance capacity (ORAC) using fluorescein as the fluorescence probe: First action 2012.23
Authors: Ou, B.
Chang, T.
Huang, D. 
Prior, R.L.
Issue Date: Nov-2013
Citation: Ou, B., Chang, T., Huang, D., Prior, R.L. (2013-11). Determination of total antioxidant capacity by oxygen radical absorbance capacity (ORAC) using fluorescein as the fluorescence probe: First action 2012.23. Journal of AOAC International 96 (6) : 1372-1376. ScholarBank@NUS Repository. https://doi.org/10.5740/jaoacint.13-175
Abstract: An improved method for the measurement of oxygen radical absorbance capacity (ORAC) was developed and validated using fluorescein (3′,6′-dihydroxyspiro[isobenzofuran-1[3H], 9′[9H]- xanthen]-3-one) as a new fluorescence probe (ORACFL). Randomly methylated β-cyclodextrin (RMCD) was introduced as the water-solubility enhancer for lipophilic antioxidants. 7% RMCD (w/v) in 50% acetone.H2O mixture sufficiently solubilized vitamin E compounds and other lipophilic phenolic antioxidants in 75 mM phosphate buffer (pH 7.4). Results indicated that fluorescein shows excellent photostability under the plate reader conditions. This ORACFL was validated through linearity, precision, accuracy, and ruggedness. The validation results demonstrated that the ORACFL assay is reliable and robust. The mean of intraday and interday CVs were
Source Title: Journal of AOAC International
URI: http://scholarbank.nus.edu.sg/handle/10635/93562
ISSN: 10603271
DOI: 10.5740/jaoacint.13-175
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