Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/93310
Title: Chromatographic analysis of low-molecular-mass copper-binding ligands from the crab species Syclla serrata and Portunis pelagicus
Authors: Ang, S.-G. 
Thi Wong, V.W. 
Issue Date: 22-May-1992
Source: Ang, S.-G.,Thi Wong, V.W. (1992-05-22). Chromatographic analysis of low-molecular-mass copper-binding ligands from the crab species Syclla serrata and Portunis pelagicus. Journal of Chromatography A 599 (1-2) : 21-24. ScholarBank@NUS Repository.
Abstract: Two copper-binding proteins and a zinc-binding ligand were isolated from the hepatopancreas of the crab Portunus pelagicus. The copper-binding proteins behave similarly to those from the crabs Carcinus maenas and Scylla serrata, and were shown to be metallothioneins. Reversed-phase high-performance liquid chromatographic (HPLC) analysis confirmed the relative purity of both proteins with only cross-contamination between the two different forms of metallothioneins, and offers a good method to separate the two forms of metallothioneins. The vast difference in the retention times (and hence the hydrophobicity) in reversed-phase HPLC indicates that the two proteins could be conformationally very different. © 1992.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/93310
ISSN: 00219673
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

58
checked on Feb 16, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.