Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF02278593
Title: Liquid chromatographic studies for essential fatty acids on a commercial alkyl phenyl bonded silica column
Authors: Ching, C.B. 
Hidajat, K. 
Rao, M.S.
Keywords: Column liquid chromatography
Direct injection
Enthalpy of adsorption
Polyunsaturated fatty acids
Issue Date: Apr-1993
Citation: Ching, C.B., Hidajat, K., Rao, M.S. (1993-04). Liquid chromatographic studies for essential fatty acids on a commercial alkyl phenyl bonded silica column. Chromatographia 35 (7-8) : 399-402. ScholarBank@NUS Repository. https://doi.org/10.1007/BF02278593
Abstract: Retention studies on arachidonic acid 5, 8, 11, 14 all cis eicosatetraenoic acid, C20:4ω6), eicosapentaenoic acid (5, 8, 11, 14, 17 all cis eicosapentaenoic acid, C20:5ω3) and docosahexaenoic acid (4, 7, 10, 13, 16, 19 all cis docosahexaenoic acid C22:6ω3) were performed on a commercial μBondapak free fatty acid analysis column. The ternary mobile phase consisting of acetonitrile, water and tetrahydrofuran was used in an isocratic mode with differential refractometry detection. Retention data were mesured at various flow rates with two different, mobile-phase compositions. Capacity factors and enthalpy of adsorption were calculated from the retention data. Finally the retention mechanism is explained. © 1993 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
Source Title: Chromatographia
URI: http://scholarbank.nus.edu.sg/handle/10635/91540
ISSN: 00095893
DOI: 10.1007/BF02278593
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