Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/90743
Title: The specific recognition of a collagen mimetic cell-binding peptide sequence derived from type I collagen for different cell types
Authors: Khew, S.T.
Tong, Y.W. 
Issue Date: 2007
Citation: Khew, S.T.,Tong, Y.W. (2007). The specific recognition of a collagen mimetic cell-binding peptide sequence derived from type I collagen for different cell types. AIChE Annual Meeting, Conference Proceedings : -. ScholarBank@NUS Repository.
Abstract: In this study, the affinity of two different cell types towards a specific cell binding sequence (GFOGER) derived from type I collagen using peptide template (PT)-assembled collagen peptides as a model for natural collagen was examined. A series of biophysical studies, including melting curve analysis and CD spectroscopy, demonstrated the presence of stable triple-helical conformation in the PT-assembled (GPO)3-GFOGER-(GPO)3, (GPO)-GFOGER-(GPO), and (Pro-Hyp-Gly)5 solution. Biological assays, including cell adhesion, competitive inhibition, and immunofluorescence staining, revealed a correlation of triple-helical conformation with the cellular recognition of GFOGER in an integrin-specific manner. Hep3B and L929 cells displayed significant differences in the recognition of GFOGER. The result showed that one specific cell binding motif may not be sufficient to mimic the ECM microenvironment.
Source Title: AIChE Annual Meeting, Conference Proceedings
URI: http://scholarbank.nus.edu.sg/handle/10635/90743
ISBN: 9780816910229
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

25
checked on May 25, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.