Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bej.2013.12.005
Title: Simultaneous detoxification, saccharification and co-fermentation of oil palm empty fruit bunch hydrolysate for l-lactic acid production by Bacillus coagulans JI12
Authors: Ye, L.
Hudari, M.S.B.
Li, Z. 
Wu, J.C.
Keywords: Bioprocess design
Cellulose
Detoxification
Lactic acid
Simultaneous saccharification and co-fermentation
Thermophiles
Issue Date: 5-Feb-2014
Source: Ye, L., Hudari, M.S.B., Li, Z., Wu, J.C. (2014-02-05). Simultaneous detoxification, saccharification and co-fermentation of oil palm empty fruit bunch hydrolysate for l-lactic acid production by Bacillus coagulans JI12. Biochemical Engineering Journal 83 : 16-21. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bej.2013.12.005
Abstract: Bacillus coagulans JI12 was used to produce l-lactic acid from both cellulose and hemicellulose sugars of oil palm empty fruit bunch hydrolysate at 50°C without sterilization prior to fermentation. In fermentation of mixed glucose and xylose (10g/L:100g/L or 50g/L:53g/L), both sugars were simultaneously converted to l-lactic acid. B. coagulans JI12 was tolerant against up to 4g/L of furfural and 20g/L of acetate and able to metabolize furfural to 2-furoic acid. After acid hydrolysis, both the hemicellulosic and cellulosic fractions of oil palm empty fruit bunch were fermented to lactic acid in a simultaneous detoxification, saccharification and co-fermentation process supplemented with 25 FPU Cellic® CTec2 cellulase per g cellulose, yielding 80.6g/L of lactic acid with a productivity of 3.4g/L/h. Neither pre-detoxification nor separation of fermentable sugars from lignin was required. These results indicate that B. coagulans JI12 is a promising strain for l-lactic acid production from lignocellulosic biomass. © 2013 Elsevier B.V.
Source Title: Biochemical Engineering Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/90156
ISSN: 1369703X
DOI: 10.1016/j.bej.2013.12.005
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