Please use this identifier to cite or link to this item:
Title: Rapid and label-free single-nucleotide discrimination via an integrative nanoparticle-nanopore approach
Authors: Ang, Y.S.
Yung, L.-Y.L. 
Keywords: gold nanoparticle
nanoparticle assembly
real-time detection
single-nucleotide polymorphism
Issue Date: 23-Oct-2012
Citation: Ang, Y.S., Yung, L.-Y.L. (2012-10-23). Rapid and label-free single-nucleotide discrimination via an integrative nanoparticle-nanopore approach. ACS Nano 6 (10) : 8815-8823. ScholarBank@NUS Repository.
Abstract: Single-nucleotide polymorphism (SNP) is an important biomarker for disease diagnosis, treatment monitoring, and development of personalized medicine. Recent works focused primarily on ultrasensitive detection, while the need for rapid and label-free single-nucleotide discrimination techniques, which are crucial criteria for translation into clinical applications, remains relatively unexplored. In this work, we developed a novel SNP detection assay that integrates two complementary nanotechnology systems, namely, a highly selective nanoparticle-DNA detection system and a single-particle sensitive nanopore readout platform, for rapid detection of single-site mutations. Discrete nanoparticle-DNA structures formed in the presence of perfectly matched (PM) or single-mismatched (SM) targets exhibited distinct size differences, which were resolved on a size-tunable nanopore platform to generate corresponding "yes/no" readout signals. Leveraging the in situ reaction monitoring capability of the nanopore platform, we demonstrated that real-time single-nucleotide discrimination of a model G487A mutation, responsible for glucose-6-phosphate dehydrogenase deficiency, can be achieved within 30 min with no false positives. Semiquantification of DNA samples down to picomolar concentration was carried out using a simple parameter of particle count without the need for sample labeling or signal amplification. The unique combination of nanoparticle-based detection and nanopore readout presented in this work brings forth a rapid, specific, yet simple biosensing strategy that can potentially be developed for point-of-care application. © 2012 American Chemical Society.
Source Title: ACS Nano
ISSN: 19360851
DOI: 10.1021/nn302636z
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Dec 12, 2018


checked on Dec 12, 2018

Page view(s)

checked on Nov 23, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.