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|Title:||Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity|
|Authors:||Qin, W.J. |
|Citation:||Qin, W.J., Yung, L.Y.L. (2007-09). Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity. Nucleic Acids Research 35 (17) : -. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkm602|
|Abstract:||Sequence-specific DNA detection is important in various biomedical applications such as gene expression profiling, disease diagnosis and treatment, drug discovery and forensic analysis. Here we report a gold nanoparticle-based method that allows DNA detection and quantification and is capable of single nucleotide polymorphism (SNP) discrimination. The precise quantification of single-stranded DNA is due to the formation of defined nanoparticle-DNA conjugate groupings in the presence of target/linker DNA. Conjugate groupings were characterized and quantified by gel electrophoresis. A linear correlation between the amount of target DNA and conjugate groupings was found. For SNP detection, single base mismatch discrimination was achieved for both the end- and center-base mismatch. The method described here may be useful for the development of a simple and quantitative DNA detection assay. © 2007 The Author(s).|
|Source Title:||Nucleic Acids Research|
|Appears in Collections:||Staff Publications|
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