Please use this identifier to cite or link to this item:
|Title:||Mannose-substituted conjugated polyelectrolyte and oligomer as an intelligent energy transfer pair for label-free visual detection of concanavalin A|
|Authors:||Pu, K.-Y. |
|Citation:||Pu, K.-Y., Shi, J., Wang, L., Cai, L., Wang, G., Liu, B. (2010-12-14). Mannose-substituted conjugated polyelectrolyte and oligomer as an intelligent energy transfer pair for label-free visual detection of concanavalin A. Macromolecules 43 (23) : 9690-9697. ScholarBank@NUS Repository. https://doi.org/10.1021/ma1021264|
|Abstract:||Mannose-substituted blue-fluorescent cationic conjugated polyelectrolyte (P1) and yellow- fluorescent neutral conjugated oligomer (6) are synthesized via the combination of Suzuki coupling polymerization and click chemistry. The spectral overlap between the emission of P1 and the absorption of 6 allows one to blend them to form an intelligent fluorescence resonance energy transfer (FRET) pair for multicolor protein sensing. Despite the nonspecific-interaction- induced perturbation in the polymer emission, strong specific binding between mannose and Concanavalin A (ConA) is able to result in protein-selective FRET from P1 to 6. The fluorescence of 6/P1 blend in the presence of ConA is dominated by yellow emission, while it remains blue in the presence of other six nonspecific proteins. The 6/P1 blend thus serves as a multicolor bicomponent FRET probe for label-free visual detection of ConA in a high-contrast and convenient manner. In addition, its linear ratiometric fluorescence response toward ConA enables effective protein quantification with a low limit of detection of ∼1.5 nM. This study demonstrates the importance of molecular engineering in conjugated polyelectrolyte based label-free chemical and biological sensing. © 2010 American Chemical Society.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 16, 2018
WEB OF SCIENCETM
checked on Oct 8, 2018
checked on Oct 12, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.