Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jconrel.2009.04.027
Title: Lysine-based peptide-functionalized PLGA foams for controlled DNA delivery
Authors: Nie, H. 
Khew, S.T.
Lee, L.Y. 
Poh, K.L.
Tong, Y.W. 
Wang, C.-H. 
Keywords: DNA delivery
Lysine peptide
Poly(d,l-lactic-co-glycolic acid)
Supercritical CO2 foaming
Issue Date: 19-Aug-2009
Citation: Nie, H., Khew, S.T., Lee, L.Y., Poh, K.L., Tong, Y.W., Wang, C.-H. (2009-08-19). Lysine-based peptide-functionalized PLGA foams for controlled DNA delivery. Journal of Controlled Release 138 (1) : 64-70. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jconrel.2009.04.027
Abstract: Due to its hydrophobicity and negatively charged surfaces, PLGA-based scaffolds have encountered problems in controlled-release and tissue engineering applications. The effects of charge modification of PLGA micro-porous foams on DNA delivery and DNA transfection are investigated herein. Tailor-designed l-lysine peptides (K4 and K20) were employed to modify the surface charge of PLGA foams using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide cross linkers and the effects of charge modification of PLGA were examined in three main aspects: DNA adsorption, DNA release properties and DNA transfection. Successful conjugation of peptide and DNA adsorption were verified by X-ray photoelectron spectroscopy. A plasmid encoding bone morphogenetic protein-2 (BMP2) was used throughout the current study and the results indicate that adsorption capacity and release behavior of DNA were highly dependent on the charge properties of the foam surfaces. The release rates of DNA from the K4- and K20-functionalized foams are more sustainable as compared to the blank foam. As a result, the sustained release of DNA from modified foams led to negligible cytotoxicity and sustained expression of DNA which is favorable for DNA delivery and tissue engineering application. Furthermore, the ease of fabrication and modification of PLGA foams makes it a promising DNA delivery device. © 2009 Elsevier B.V. All rights reserved.
Source Title: Journal of Controlled Release
URI: http://scholarbank.nus.edu.sg/handle/10635/89345
ISSN: 01683659
DOI: 10.1016/j.jconrel.2009.04.027
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

12
checked on Jul 22, 2018

WEB OF SCIENCETM
Citations

11
checked on Jun 19, 2018

Page view(s)

31
checked on Jul 6, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.