Please use this identifier to cite or link to this item:
|Title:||Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells|
|Citation:||Yu, H., Lui, Y.S., Xiong, S., Leong, W.S., Wen, F., Nurkahfianto, H., Rana, S., Leong, D.T., Ng, K.W., Tan, L.P. (2013-01-01). Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells. Stem Cells and Development 22 (1) : 136-147. ScholarBank@NUS Repository. https://doi.org/10.1089/scd.2012.0160|
|Abstract:||We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β3 integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering. © 2013, Mary Ann Liebert, Inc.|
|Source Title:||Stem Cells and Development|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2018
WEB OF SCIENCETM
checked on Apr 24, 2018
checked on May 18, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.