Please use this identifier to cite or link to this item: https://doi.org/10.1089/scd.2012.0160
Title: Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells
Authors: Yu, H.
Lui, Y.S.
Xiong, S.
Leong, W.S.
Wen, F.
Nurkahfianto, H.
Rana, S.
Leong, D.T. 
Ng, K.W.
Tan, L.P.
Issue Date: 1-Jan-2013
Source: Yu, H., Lui, Y.S., Xiong, S., Leong, W.S., Wen, F., Nurkahfianto, H., Rana, S., Leong, D.T., Ng, K.W., Tan, L.P. (2013-01-01). Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells. Stem Cells and Development 22 (1) : 136-147. ScholarBank@NUS Repository. https://doi.org/10.1089/scd.2012.0160
Abstract: We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β3 integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering. © 2013, Mary Ann Liebert, Inc.
Source Title: Stem Cells and Development
URI: http://scholarbank.nus.edu.sg/handle/10635/89241
ISSN: 15473287
DOI: 10.1089/scd.2012.0160
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

25
checked on Feb 21, 2018

WEB OF SCIENCETM
Citations

19
checked on Jan 15, 2018

Page view(s)

38
checked on Feb 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.