Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2008.02.047
Title: High-affinity sulfonated materials with transition metal counterions for enhanced protein separation in dual-layer hollow fiber membrane chromatography
Authors: Li, Y. 
Chung, T.-S. 
Chan, S.Y. 
Keywords: Affinity
Coordination
Dual-layer hollow fiber
Protein separation
Transition metal ions
Issue Date: 11-Apr-2008
Citation: Li, Y., Chung, T.-S., Chan, S.Y. (2008-04-11). High-affinity sulfonated materials with transition metal counterions for enhanced protein separation in dual-layer hollow fiber membrane chromatography. Journal of Chromatography A 1187 (1-2) : 285-288. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2008.02.047
Abstract: High-affinity membrane materials have been successfully synthesized through a combination of the polymer sulfonation reaction with transition metal counterion exchange treatment. This type of promising materials were embodied for the first time with the aid of dual-layer hollow fiber technology for protein separation. Three types of immobilized metal affinity membranes (Cu2+, Ni2+ and Zn2+ forms) were developed in this work and they all exhibited enhanced protein separation performance compared to the as-spun hollow fiber in H+ form due to the strong affinity between transition metal counterions and target protein molecules. Ultimately, the high-purity target protein (>99%, w/w) could be achieved via the membrane in Cu2+ form. © 2008 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/89089
ISSN: 00219673
DOI: 10.1016/j.chroma.2008.02.047
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