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Title: Gold nanostructures for the multiplex detection of glucose-6-phosphate dehydrogenase gene mutations
Authors: Seow, N.
Lai, P.S.
Yung, L.-Y.L. 
Keywords: Gel electrophoresis
Glucose-6-phosphate dehydrogenase (G6PD) deficiency
Gold nanoparticles
Multiplex detection
Mutation screening
Issue Date: 15-Apr-2014
Citation: Seow, N., Lai, P.S., Yung, L.-Y.L. (2014-04-15). Gold nanostructures for the multiplex detection of glucose-6-phosphate dehydrogenase gene mutations. Analytical Biochemistry 451 (1) : 56-62. ScholarBank@NUS Repository.
Abstract: We describe a gold nanoparticle-based technique for the detection of single-base mutations in the glucose-6-phosphate dehydrogenase (G6PD) gene, a condition that can lead to neonatal jaundice and hemolytic anemia. The aim of this technique is to clearly distinguish different mutations frequently described within the Asian population from their wild-type counterparts and across different mutant variants. Gold nanoparticles of different sizes were synthesized, and each was conjugated with a single-strand DNA (ssDNA) sequence specific for a particular mutation in the G6PD gene. It was found that only mutant targets presented a characteristic band on the agarose gel, indicating the successful formation of dimeric nanostructures. No such dimer bands were observed for the wild-type targets. The difference in the relative dimer band levels allowed different mutant variants to be distinguished from one another. The technique was further validated using G6PD-deficient patient samples. This simple mutation detection method with direct result readout is amenable for rapid and mass screening of samples. © 2014 Elsevier Inc. All rights reserved.
Source Title: Analytical Biochemistry
ISSN: 10960309
DOI: 10.1016/j.ab.2014.01.014
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