Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.24632
Title: Engineering of recombinant E. coli cells co-expressing P450pyrTM monooxygenase and glucose dehydrogenase for highly regio- and stereoselective hydroxylation of alicycles with cofactor recycling
Authors: Pham, S.Q.
Gao, P.
Li, Z. 
Keywords: Cofactor regeneration
Enantioselectivity
Hydroxylation
P450 monooxygenase
Regioselectivity
Whole-cell biocatalysis
Issue Date: Feb-2013
Source: Pham, S.Q., Gao, P., Li, Z. (2013-02). Engineering of recombinant E. coli cells co-expressing P450pyrTM monooxygenase and glucose dehydrogenase for highly regio- and stereoselective hydroxylation of alicycles with cofactor recycling. Biotechnology and Bioengineering 110 (2) : 363-373. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.24632
Abstract: E. coli (P450pyrTM-GDH) with dual plasmids, pETDuet containing P450pyr triple mutant I83H/M305Q/A77S (P450pyrTM) and ferredoxin reductase (FdR) genes and pRSFDuet containing glucose dehydrogenase (GDH) and ferredoxin (Fdx) genes, was engineered to show a high activity (12.7Ug-1cdw) for the biohydroxylation of N-benzylpyrrolidine 1 and a GDH activity of 106Ug-1 protein. The E. coli cells were used as efficient biocatalysts for highly regio- and stereoselective hydroxylation of alicyclic substrates at non-activated carbon atom with enhanced productivity via intracellular recycling of NAD(P)H. Hydroxylation of N-benzylpyrrolidine 1 with resting cells in the presence of glucose showed excellent regio- and stereoselectivity, giving (S)-N-benzyl-3-hydroxypyrrolidine 2 in 98% ee as the sole product in 9.8mM. The productivity is much higher than that of the same biohydroxylation using E. coli (P450pyrTM)b without expressing GDH. E. coli (P450pyrTM-GDH) was found to be highly regio- and stereoselective for the hydroxylation of N-benzylpyrrolidin-2-one 3, improving the regioselectivity from 90% of the wild-type P450pyr to 100% and giving (S)-N-benzyl-4-hydroxylpyrrolidin-2-one 4 in 99% ee as the sole product. A high activity of 15.5Ug-1cdw was achieved and (S)-4 was obtained in 19.4mM. E. coli (P450pyrTM-GDH) was also found to be highly regio- and stereoselective for the hydroxylation of N-benzylpiperidin-2-one 5, increasing the ee of the product (S)-N-benzyl-4-hydroxy-piperidin-2-one 6 to 94% from 33% of the wild-type P450pyr. A high activity of 15.8Ug-1cdw was obtained and (S)-6 was produced in 3.3mM as the sole product. E. coli (P450pyrTM-GDH) represents the most productive system known thus far for P450-catalyzed hydroxylations with cofactor recycling, and the hydroxylations with E. coli (P450pyrTM-GDH) provide with simple and useful syntheses of (S)-2, (S)-4, and (S)-6 that are valuable pharmaceutical intermediates and difficult to prepare. © 2012 Wiley Periodicals, Inc.
Source Title: Biotechnology and Bioengineering
URI: http://scholarbank.nus.edu.sg/handle/10635/88842
ISSN: 00063592
DOI: 10.1002/bit.24632
Appears in Collections:Staff Publications

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