Please use this identifier to cite or link to this item: https://doi.org/10.1089/ten.tea.2007.0346
Title: Delivery of basic fibroblast growth factor from gelatin microsphere scaffold for the growth of human umbilical vein endothelial cells
Authors: Zhu, X.H. 
Tabata, Y.
Wang, C.-H. 
Tong, Y.W. 
Issue Date: 1-Nov-2008
Citation: Zhu, X.H., Tabata, Y., Wang, C.-H., Tong, Y.W. (2008-11-01). Delivery of basic fibroblast growth factor from gelatin microsphere scaffold for the growth of human umbilical vein endothelial cells. Tissue Engineering - Part A 14 (12) : 1939-1947. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tea.2007.0346
Abstract: One of the major obstacles for engineering large tissue or organs such as the liver in vitro is the insufficient supply of nutrients and oxygen to the cells growing inside the scaffold, which reduces cell viability significantly. Therefore, vascularization of the scaffolding system is necessary for successful engineering of such tissues. In this study, we investigated the use of gelatin microsphere as scaffold to culture human umbilical vein endothelial cells, which is considered to be the basis and premise for the formation of blood vessels. The gelatin microspheres were crosslinked with different concentrations of glutaraldehyde to study the effects of crosslinking extent on the growth of endothelial cells. The swelling ratios of the gelatin microspheres decreased from 5.9 ± 0.8 to 3.9 ± 0.6 with the increase of the crosslinking extent. Basic fibroblast growth factors (bFGFs), which can improve endothelial cell proliferation as well as stimulate the formation of capillary vessels, were incorporated into the gelatin microspheres through ionic complexation. Sustained delivery of the growth factors was achieved for at least 2 weeks. The proliferation of the cells cultured on the bFGF-encapsulated microspheres was improved by about two times as compared to control and about 1.3 times as compared to blank microspheres, which indicated that the bioactivity of bFGF was well maintained, and the delivery of the growth factors directly to the cells significantly improved the success of this tissue engineering system. © Copyright 2008, Mary Ann Liebert, Inc.
Source Title: Tissue Engineering - Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/88736
ISSN: 19373341
DOI: 10.1089/ten.tea.2007.0346
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

39
checked on May 20, 2018

WEB OF SCIENCETM
Citations

37
checked on Apr 18, 2018

Page view(s)

39
checked on May 11, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.