Please use this identifier to cite or link to this item: https://doi.org/10.1002/adhm.201200243
Title: Conjugated Polymer Amplified Far-Red/Near-Infrared Fluorescence from Nanoparticles with Aggregation-Induced Emission Characteristics for Targeted In Vivo Imaging
Authors: Ding, D. 
Li, K.
Qin, W.
Zhan, R.
Hu, Y.
Liu, J.
Tang, B.Z.
Liu, B. 
Keywords: Aggregation-induced emission
Conjugated polymer
Fluorescence resonance energy transfer
In vivo fluorescence imaging
Targeted imaging
Issue Date: Mar-2013
Citation: Ding, D., Li, K., Qin, W., Zhan, R., Hu, Y., Liu, J., Tang, B.Z., Liu, B. (2013-03). Conjugated Polymer Amplified Far-Red/Near-Infrared Fluorescence from Nanoparticles with Aggregation-Induced Emission Characteristics for Targeted In Vivo Imaging. Advanced Healthcare Materials 2 (3) : 500-507. ScholarBank@NUS Repository. https://doi.org/10.1002/adhm.201200243
Abstract: Fluorescence-amplified far-red/near-infrared (FR/NIR) nanoparticles (NPs) are synthesized by co-encapsulation of conjugated polymer donor (poly[9,9-bis(2-(2-(2-methoxyethoxy)ethoxy)ethyl)fluorenyldivinylene]; PFV) and a fluorogen acceptor (2-(2,6-bis((E)-4-(phenyl(4′-(1,2,2-triphenylvinyl)-[1,1′-biphenyl]-4-yl)amino)styryl)-4H-pyran-4-ylidene)malononitrile; TPE-TPA-DCM) with aggregation-induced emission (AIE) characteristics using biocompatible bovine serum albumin (BSA) as the encapsulation matrix. The good spectral overlap and close proximity between PFV and TPE-TPA-DCM in BSA NPs result in a 5.3-fold amplified TPE-TPA-DCM emission signal via fluorescence resonance energy transfer (FRET). The obtained PFV/TPE-TPA-DCM co-loaded BSA NPs are spherical in shape with a large Stokes shift of ∼223 nm and low cytotoxicity. The BSA matrix allows further functionalization with arginine-glycine-aspartic acid (RGD) peptide to yield fluorescent probes for specific recognition of integrin receptor-overexpressed cancer cells. The advantage of PFV amplified FR/NIR signal from TPE-TPA-DCM is further demonstrated in cellular and in vivo imaging using HT-29 colon cancer cells and a murine hepatoma H22 tumor-bearing mouse model, respectively. The high FR/NIR fluorescence and specific cancer targeting ability by RGD surface functionalization make the PFV/TPE-TPA-DCM co-loaded BSA-RGD NPs a unique FR/NIR fluorescent probe for cellular imaging and in vivo tumor diagnosis in a high contrast and selective manner. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Advanced Healthcare Materials
URI: http://scholarbank.nus.edu.sg/handle/10635/88688
ISSN: 21922640
DOI: 10.1002/adhm.201200243
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