Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.snb.2013.01.036
Title: A liquid crystal biosensor for detecting organophosphates through the localized pH changes induced by their hydrolytic products
Authors: Chen, C.-H. 
Yang, K.-L. 
Keywords: Biosensors
Liquid crystal
Organophosphate
Paraoxonase 1
Issue Date: 2013
Citation: Chen, C.-H., Yang, K.-L. (2013). A liquid crystal biosensor for detecting organophosphates through the localized pH changes induced by their hydrolytic products. Sensors and Actuators, B: Chemical 181 : 368-374. ScholarBank@NUS Repository. https://doi.org/10.1016/j.snb.2013.01.036
Abstract: We report a biosensor for detecting organophosphates (OPs) by using liquid crystal (LC). The mechanism of the biosensor is based on detection of minute pH changes during the enzymatic hydrolysis of OPs. To hydrolyze OPs, paraoxonase 1 (PON1) is immobilized on a copper grid which also hosts pH-sensitive LC, 4-cyano-4′-pentylbiphenyl (5CB) doped with 0.3% of 4′-pentyl- biphenyl-4-carboxylic acid (PBA). Proximity of enzyme and LC ensures that H + can be detected by the pH-sensitive LC before it is neutralized by buffer. Thus, the presence of OPs can be reported as color change in LC when they undergo enzymatic hydrolysis. For paraoxon detection, we can reach a limit of detection (LOD) of 1 μM by using the naked eye. This type of LC-based biosensor also shows high specificity and does not respond to imidacloprid and ampicillin. © 2013 Elsevier B.V.
Source Title: Sensors and Actuators, B: Chemical
URI: http://scholarbank.nus.edu.sg/handle/10635/88455
ISSN: 09254005
DOI: 10.1016/j.snb.2013.01.036
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