Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.colsurfb.2007.05.019
Title: Real time observation of diffusion and bioaffinity binding processes in single polyelectrolyte-coated microcapsules: A fluorescence-based approach
Authors: Mak, W.C. 
Yangzhong, H.
Trau, D. 
Keywords: Biotin avidin
Layer-by-layer
Microcapsule diffusion
Polyelectrolyte
Issue Date: 15-Oct-2007
Source: Mak, W.C., Yangzhong, H., Trau, D. (2007-10-15). Real time observation of diffusion and bioaffinity binding processes in single polyelectrolyte-coated microcapsules: A fluorescence-based approach. Colloids and Surfaces B: Biointerfaces 60 (1) : 125-130. ScholarBank@NUS Repository. https://doi.org/10.1016/j.colsurfb.2007.05.019
Abstract: We report on using fluorescence microscopy to study, visualize and determine the diffusion phenomena into and bioaffinity binding within single microcapsules in real time by using biotin-fluorescein as diffusive species and encapsulated avidin as binding partner. Microcapsules were constructed by entrapment of avidin within an agarose matrix and encapsulated with polyelectrolyte layers by Layer-by-Layer (LbL) polyelectrolyte self assembly. A "ring" of high fluorescence intensity advancing with time towards the capsule centre was observed during incubation of capsules with fluorescent-labeled biotin. Fluorescence intensity was build up in capsule areas where binding to avidin occurred and was visualized in real time. A model for the diffusion process in microcapsules was developed and experimental data was plotted and fitted well with trends predicted by the model. The value of the diffusion coefficient for biotin-fluorescein was determined to be 3.5 × 10-8 cm2/s, which is comparable to literature values of similar sized molecules. © 2007 Elsevier B.V. All rights reserved.
Source Title: Colloids and Surfaces B: Biointerfaces
URI: http://scholarbank.nus.edu.sg/handle/10635/88085
ISSN: 09277765
DOI: 10.1016/j.colsurfb.2007.05.019
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