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Title: Force-dependent vinculin binding to talin in live cells: A crucial step in anchoring the actin cytoskeleton to focal adhesions
Authors: Hirata, H.
Tatsumi, H.
Lim, C.T. 
Sokabe, M.
Keywords: Focal adhesion
Molecular clutch
Issue Date: 15-Mar-2014
Citation: Hirata, H., Tatsumi, H., Lim, C.T., Sokabe, M. (2014-03-15). Force-dependent vinculin binding to talin in live cells: A crucial step in anchoring the actin cytoskeleton to focal adhesions. American Journal of Physiology - Cell Physiology 306 (6) : C607-C620. ScholarBank@NUS Repository.
Abstract: Mechanical forces play a pivotal role in the regulation of focal adhesions (FAs where the actin cytoskeleton is anchored to the extracellular matrix through integrin and a variety of linker proteins including talin and vinculin. The localization of vinculin at FAs depends on mechanical forces. While in vitro studies have demonstrated the force-induced increase in vinculin binding to talin, it remains unclear whether such a mechanism exists at FAs in vivo. In this study, using fibroblasts cultured on elastic silicone substrata, we have examined the role of forces in modulating talin-vinculin binding at FAs. Stretching the substrata caused vinculin accumulation at talin-containing FAs, and this accumulation was abrogated by expressing the talin-binding domain of vinculin (domain D1, which inhibits endogenous vinculin from binding to talin. These results indicate that mechanical forces loaded to FAs facilitate vinculin binding to talin at FAs. In cell-protruding regions, the actin network moved backward over talin-containing FAs in domain D1-expressing cells while it was anchored to FAs in control cells, suggesting that the force-dependent vinculin binding to talin is crucial for anchoring the actin cytoskeleton to FAs in living cells. © 2014 the American Physiological Society.
Source Title: American Journal of Physiology - Cell Physiology
ISSN: 15221563
DOI: 10.1152/ajpcell.00122.2013
Appears in Collections:Staff Publications

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