Please use this identifier to cite or link to this item:
Title: Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering
Authors: Jin, G.
Prabhakaran, M.P. 
Ramakrishna, S. 
Keywords: Epidermal lineage
Induction media
Stem cell
Issue Date: Aug-2011
Citation: Jin, G., Prabhakaran, M.P., Ramakrishna, S. (2011-08). Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering. Acta Biomaterialia 7 (8) : 3113-3122. ScholarBank@NUS Repository.
Abstract: Bone marrow (BM) mesenchymal stem cells (MSC) capable of differentiating along the epidermal lineage on engineered nanofibrous scaffolds have great potential for bionanomaterial-cell transplantation therapy of skin wounds. MSC have been the focus of many tissue engineering studies, mainly because of their multipotential properties. We investigated the potential of human BM-derived MSC for epidermal cell differentiation in vitro on electrospun collagen/poly(l- lactic acid)-co-poly(3-caprolactone) (Coll/PLLCL) nanofibrous scaffolds. PLLCL and Coll/PLLCL nanofibrous scaffolds were fabricated by an electrospinning process and their chemical and mechanical characterization carried out by scanning electron microscopy (SEM), water contact angle determination, Fourier transform infrared spectroscopy, and tensile testing. The differentiation of MSC was carried out using epidermis inducing factors, including epidermal growth factor (EGF) and 1,25-dihydroxyvitamin D3, in culture medium. The proliferation of MSC evaluated by cell proliferation assay showed that the number of cells grown on Coll/PLLCL nanofibrous scaffolds was significantly higher than those on PLLCL scaffolds. The SEM results showed that MSC differentiated on Coll/PLLCL nanofibrous scaffolds showed a round keratinocyte morphology and expressed keratin 10, filaggrin and partial involucrin protein by immunofluorescent microscopic studies. The interaction of MSC and nanofibers was studied and we concluded that the electrospun Coll/PLLCL nanofibers could mimic the native skin extracellular matrix environment and are promising substrates for advanced skin tissue engineering. Our studies on the differentiation of MSC along the epidermal lineage on nanofibrous scaffolds suggest their potential application in skin regeneration without regional differentiation. © 2011 Acta Materialia Inc.
Source Title: Acta Biomaterialia
ISSN: 17427061
DOI: 10.1016/j.actbio.2011.04.017
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 19, 2019


checked on Mar 19, 2019

Page view(s)

checked on Mar 16, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.