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|Title:||Photosensitive and biomimetic core-shell nanofibrous scaffolds as wound dressing|
|Citation:||Jin, G., Prabhakaran, M.P., Ramakrishna, S. (2014). Photosensitive and biomimetic core-shell nanofibrous scaffolds as wound dressing. Photochemistry and Photobiology 90 (3) : 673-681. ScholarBank@NUS Repository. https://doi.org/10.1111/php.12238|
|Abstract:||Tissue engineered skin grafts that mimic the native extracellular matrix of skin has gained huge popularity among clinicians since they increase the survival rate of the patients. Phototherapy shows promising results with respect to acute and chronic pain relief, treatment of inflammatory conditions and promotion of wound healing. Here, we encapsulated a photosensitive polymer poly (3-hexylthiophene) (P3HT) and epidermal growth factor in the core-shell-structured Gelatin/poly(L-lactic acid)-co-poly-(ε-caprolactone) nanofibers [Gel/PLLCL/P3GF(cs)] by coaxial spinning and studied the potential application of the Gel/PLLCL/P3GF(cs) nanofibrous scaffold as a novel skin graft. The proliferation of fibroblasts was significantly improved on Gel/PLLCL/P3GF(cs) under light stimulation compared to fibroblasts on the same scaffold under dark condition. Studies on the in vitro wound healing ability of Gel/PLLCL/P3GF(cs) showed complete closure of wound after 9 days under "light stimulation" too. Furthermore, the potential of adipose-derived stem cells (ASCs) to differentiate to epidermal cells on Gel/PLLCL/P3GF(cs) was evaluated. The differentiated ASCs with keratinocytes morphology were only found on the light stimulated Gel/PLLCL/P3GF(cs). Our results suggest that the photosensitive core-shell Gel/PLLCL/P3GF(cs) nanofibers could be a novel substrate to aid in the reestablishment of skin architecture. A photosensitive polymer, namely, poly (3-hexylthiophene) is encapsulated within the core-shell-structured Gel/PLLCL fibers by coaxial electrospinning. The proliferation of human dermal fibroblasts was significantly improved on the core-shell nanofibers under light stimulation compared to fibroblasts on the same scaffold under nonstimulated condition. Moreover, the stem cells on the photosensitive scaffolds under light stimulation were more likely to differentiate into epidermal lineages compared to cells without stimulation. Our results suggest that the photosensitive core-shell nanofibers can serve as a novel substrate to promote skin regeneration. © 2014 The American Society of Photobiology.|
|Source Title:||Photochemistry and Photobiology|
|Appears in Collections:||Staff Publications|
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