Please use this identifier to cite or link to this item: https://doi.org/10.1039/c3tb21789b
Title: Cross-linking of protein scaffolds for therapeutic applications: PCL nanofibers delivering riboflavin for protein cross-linking
Authors: Sridhar, R.
Madhaiyan, K.
Sundarrajan, S. 
Góra, A.
Venugopal, J.R. 
Ramakrishna, S. 
Issue Date: 28-Mar-2014
Citation: Sridhar, R., Madhaiyan, K., Sundarrajan, S., Góra, A., Venugopal, J.R., Ramakrishna, S. (2014-03-28). Cross-linking of protein scaffolds for therapeutic applications: PCL nanofibers delivering riboflavin for protein cross-linking. Journal of Materials Chemistry B 2 (12) : 1626-1633. ScholarBank@NUS Repository. https://doi.org/10.1039/c3tb21789b
Abstract: Nanofibers play a significant role in tissue engineering and drug delivery because of the ease with which drugs or pharmaceuticals may be incorporated into the nano-formulation. Natural protein nanofibers are cross-linked (CXLed) employing a new protocol to improve their stability for perspective usage as tissue engineering or drug delivery scaffolds. The protocol utilizes a non-toxic, natural material vitamin based CXL protocol that works well for stabilizing protein nanofibers. We have tested the generation of reactive oxygen species (ROS) from UV treated riboflavin-gelatin microfibers, film or solution that helps in gelatin (Gel) CXLing and results in improved mechanical properties. Further natural proteins Gel and fibrinogen (Fib) solutions were also CXLed using vitamin B2 (riboflavin (Rib)) released from Rib-loaded polycaprolactone (PCL) nanofibers followed by UV treatment. The sustained release of Rib from PCL nanofibers is studied with in vitro drug release experiments and in vitro hydrogel formation upon treatment with the natural protein solutions. Rib-loaded nanofibers were characterized with SEM and AFM for morphology, mechanical strength calculation and FT-IR for ensuring drug incorporation. The Rib encapsulation in the nanofiber reservoirs enables the sustained release, and the ROS generating nanofibers could find application as a patch for CXLing any protein fiber or fibrous tissue, such as ocular, skin or cardiac tissue engineering. © The Royal Society of Chemistry 2014.
Source Title: Journal of Materials Chemistry B
URI: http://scholarbank.nus.edu.sg/handle/10635/84948
ISSN: 20507518
DOI: 10.1039/c3tb21789b
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

17
checked on Sep 22, 2018

WEB OF SCIENCETM
Citations

14
checked on Sep 12, 2018

Page view(s)

78
checked on Sep 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.