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|Title:||Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells|
|Authors:||Zhu, L. |
|Source:||Zhu, L., Zhang, Q., Feng, H., Ang, S., Chau, F.S., Liu, W.-T. (2004-08). Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells. Lab on a Chip - Miniaturisation for Chemistry and Biology 4 (4) : 337-341. ScholarBank@NUS Repository. https://doi.org/10.1039/b401834f|
|Abstract:||A filter-based microfluidic device was combined with immunofluorescent labeling as a platform to rapidly detect microbial cells. The coin-sized device consisted of micro-chambers, micro-channels and filter weirs (gap = 1-2 μm), and was demonstrated to effectively trap and concentrate microbial cells (i.e., Cryptosporidium parvum and Giardia lamblia), which were larger in size than the weir gap. After sample injection, a staining solution containing fluorescently-labeled antibodies was continuously provided into the device (flow rate = 20 μl min-1) to flush the microbial cells toward the weirs and to accelerate the fluorescent labeling reaction. Using a staining solution that was 10 to 100 times more dilute than the recommended concentration used in a conventional glass method, those target cells with a fluorescent signal-to-noise ratio of 12 could be microscopically observed at single-cell level within 2 to 5 min prior to secondary washing.|
|Source Title:||Lab on a Chip - Miniaturisation for Chemistry and Biology|
|Appears in Collections:||Staff Publications|
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