Please use this identifier to cite or link to this item: https://doi.org/10.1039/b401834f
Title: Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells
Authors: Zhu, L. 
Zhang, Q.
Feng, H.
Ang, S.
Chau, F.S. 
Liu, W.-T. 
Issue Date: Aug-2004
Citation: Zhu, L., Zhang, Q., Feng, H., Ang, S., Chau, F.S., Liu, W.-T. (2004-08). Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells. Lab on a Chip - Miniaturisation for Chemistry and Biology 4 (4) : 337-341. ScholarBank@NUS Repository. https://doi.org/10.1039/b401834f
Abstract: A filter-based microfluidic device was combined with immunofluorescent labeling as a platform to rapidly detect microbial cells. The coin-sized device consisted of micro-chambers, micro-channels and filter weirs (gap = 1-2 μm), and was demonstrated to effectively trap and concentrate microbial cells (i.e., Cryptosporidium parvum and Giardia lamblia), which were larger in size than the weir gap. After sample injection, a staining solution containing fluorescently-labeled antibodies was continuously provided into the device (flow rate = 20 μl min-1) to flush the microbial cells toward the weirs and to accelerate the fluorescent labeling reaction. Using a staining solution that was 10 to 100 times more dilute than the recommended concentration used in a conventional glass method, those target cells with a fluorescent signal-to-noise ratio of 12 could be microscopically observed at single-cell level within 2 to 5 min prior to secondary washing.
Source Title: Lab on a Chip - Miniaturisation for Chemistry and Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/84595
ISSN: 14730197
DOI: 10.1039/b401834f
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