Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ab.2011.05.045
Title: Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay
Authors: Vashist, S.K.
Zhang, B.
Zheng, D.
Al-Rubeaan, K.
Luong, J.H.T.
Sheu, F.-S. 
Issue Date: Oct-2011
Citation: Vashist, S.K., Zhang, B., Zheng, D., Al-Rubeaan, K., Luong, J.H.T., Sheu, F.-S. (2011-10). Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay. Analytical Biochemistry 417 (1) : 156-158. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ab.2011.05.045
Abstract: This study revealed a major interference from sulfo-N-hydroxysuccinimide (sulfo-NHS) in the bicinchoninic acid (BCA) protein assay. Sulfo-NHS, a common reagent used in bioconjugation and analytical biochemistry, exhibited absorbance signals and absorbance peaks at 562 nm, comparable to bovine serum albumin (BSA). However, the combined absorbance of sulfo-NHS and BSA was not strictly additive. The sulfo-NHS interference was suggested to be caused by the reduction of Cu 2+ in the BCA Kit's reagent B (4% cupric sulfate) in a manner similar to that of the protein. Crown Copyright © 2011 Published by Elsevier Inc. All rights reserved.
Source Title: Analytical Biochemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/83113
ISSN: 00032697
DOI: 10.1016/j.ab.2011.05.045
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