Optimization of an advanced non-invasive light activated disinfection strategy

Abstract

A photosensitizer formulation and strategy was developed based on the photophysical, photochemical and photobiological characteristics of methylene blue (MB) for the disinfection of root canal using light activated therapy. Disinfection of matured E. faecalis biofilms on root canal dentine was tried with the newly developed 'Advanced Non-Invasive Light Activated Disinfection' (ANILAD), conventional photodynamic therapy, and conventional root canal therapy alone or in combination with AÑILAD. The results showed that , although complete disinfection of non-matured biofilm is possible by ANILAD alone, a combination of conventional root canal treatment (RCT) with ANILAD could achieve significantly higher bacterial killing (6log10-7log10 bacterial reduction) compared to any other tested treatment in matured biofilm (p