Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/79769
Title: THE ROLE OF THE FLAGELLUM ATTACHMENT ZONE IN THE ORGANIZATION OF THE MICROTUBULE CYTOSKELETON OF TRYPANOSOMA BRUCEI
Authors: OMAR SHERIFF
Keywords: Trypanosoma brucei, microtubule, cytoskeleton, morphology, organelle inheritance, organelle assembly
Issue Date: 27-Mar-2014
Source: OMAR SHERIFF (2014-03-27). THE ROLE OF THE FLAGELLUM ATTACHMENT ZONE IN THE ORGANIZATION OF THE MICROTUBULE CYTOSKELETON OF TRYPANOSOMA BRUCEI. ScholarBank@NUS Repository.
Abstract: THE MICROTUBULE CYTOSKELETON FORMS THE MOST PROMINENT STRUCTURAL SYSTEM IN TRYPANOSOMA BRUCEI, UNDERGOING EXTENSIVE MODIFICATIONS DURING THE CELL CYCLE. VISUALIZATION OF TYROSINATED MICROTUBULES LEADS TO A SEMICONSERVATIVE MODE OF INHERITANCE, WHEREAS RECENT STUDIES EMPLOYING MICROTUBULE PLUS END TRACKING PROTEINS HAVE HINTED AT AN ASYMMETRIC INHERITANCE. TO FURTHER THIS KNOWLEDGE, THE DYNAMICS OF THE MICROTUBULE CYTOSKELETON WAS VISUALIZED BY INDUCIBLE YFP-α-TUBULIN EXPRESSION. DURING NEW FLAGELLUM/FLAGELLUM ATTACHMENT ZONE (FAZ) BIOGENESIS AND CELL GROWTH, YFP-α-TUBULIN WAS INCORPORATED MAINLY BETWEEN THE OLD AND NEW FLAGELLUM/FAZ COMPLEXES. CYTOSKELETAL MODIFICATIONS AT THE POSTERIOR END OF THE CELLS WERE OBSERVED WITH EB1, A MICROTUBULE PLUS END BINDING PROTEIN, PARTICULARLY DURING MITOSIS. ADDITIONALLY, THE NEWLY FORMED MICROTUBULES SEGREGATED ASYMMETRICALLY, WITH THE DAUGHTER CELL INHERITING THE NEW FLAGELLUM/FAZ COMPLEX RETAINING MOST OF THE NEW MICROTUBULES. TOGETHER,
URI: http://scholarbank.nus.edu.sg/handle/10635/79769
Appears in Collections:Ph.D Theses (Open)

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