Microchip capillary electrophoresis.

Abstract

Microchip capillary electrophoresis (MCE) is gaining popularity due to the developments of simple microfabrication methods under nonstringent laboratory conditions. Moreover, the low material and production costs of polymer-based microchips have further stimulated advances in the applications of MCE in various fields, including clinical analysis, drug screening, biomarker identification, and biosensing. In this chapter, a simple and robust protocol for fabrication of microchips for lab-on-chip testing and microchip electrophoresis is described. The microchips are hybrid poly(dimethylsiloxane) (PDMS)/glass microchips, which are produced by a combination of photolithography and micromolding processes. This type of microchip has been used in a wide range of analyses.