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|Title:||Optical detection of photo-activated protein using Brewster angle microscopy|
|Source:||Li, Y.,Gorelik, S.,Tomczak, N.,Song, H.Y.,Lear, M.J.,Hobley, J. (2008). Optical detection of photo-activated protein using Brewster angle microscopy. 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008 : -. ScholarBank@NUS Repository. https://doi.org/10.1109/IPGC.2008.4781381|
|Abstract:||Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).|
|Source Title:||2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008|
|Appears in Collections:||Staff Publications|
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