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|Title:||Spatial temperature gradient capillary electrophoresis for DNA mutation detection|
|Authors:||Zhu, L. |
Spatial temperature gradient capillary electrophoresis
|Source:||Zhu, L.,Lee, H.K.,Lin, B.,Yeung, E.S. (2001). Spatial temperature gradient capillary electrophoresis for DNA mutation detection. Electrophoresis 22 (17) : 3683-3687. ScholarBank@NUS Repository. https://doi.org/10.1002/1522-2683(200109)22:17<3683|
|Abstract:||A continuous spatial temperature gradient was established in capillary electrophoresis by using a simple temperature control device. The temperature profile along the capillary was predicted by theoretical calculations. A nearly linear spatial temperature gradient was established and applied to DNA mutation detection. By spanning a wide temperature range, it was possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures.|
|Appears in Collections:||Staff Publications|
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