Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bmcl.2005.03.079

Title:	Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing
Authors:	Girish, A. Sun, H. Yeo, D.S.Y. Chen, G.Y.J. Chua, T.-K. Yao, S.Q.
Keywords:	Intein N-terminal cysteine-containing protein Protein array Proteomics
Issue Date:	16-May-2005
Citation:	Girish, A., Sun, H., Yeo, D.S.Y., Chen, G.Y.J., Chua, T.-K., Yao, S.Q. (2005-05-16). Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing. Bioorganic and Medicinal Chemistry Letters 15 (10) : 2447-2451. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2005.03.079
Abstract:	One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system. © 2005 Elsevier Ltd. All rights reserved.
Source Title:	Bioorganic and Medicinal Chemistry Letters
URI:	http://scholarbank.nus.edu.sg/handle/10635/76993
ISSN:	0960894X
DOI:	10.1016/j.bmcl.2005.03.079
Appears in Collections:	Staff Publications

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