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|Title:||Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing|
N-terminal cysteine-containing protein
|Citation:||Girish, A., Sun, H., Yeo, D.S.Y., Chen, G.Y.J., Chua, T.-K., Yao, S.Q. (2005-05-16). Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing. Bioorganic and Medicinal Chemistry Letters 15 (10) : 2447-2451. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2005.03.079|
|Abstract:||One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system. © 2005 Elsevier Ltd. All rights reserved.|
|Source Title:||Bioorganic and Medicinal Chemistry Letters|
|Appears in Collections:||Staff Publications|
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