Separation and determination of Stevia sweeteners by capillary electrophoresis and high performance liquid chromatography

Abstract

In this paper, the separation of steviol glycosides in stevia sweeteners including stevioside, rebaudioside A, rebaudioside C and dulcoside A by capillary electrophoresis and high performance liquid chromatography was investigated. A simple and efficient capillary electrophoretic method was developed. The results were compared with those obtained by HPLC. The individual steviol glycosides were obtained by HPLC fraction collection, and peaks in the electropherograms of the sweetener samples from Chinese refining factories were identified by comparing with those of individual steviol glycosides. The method was applied to the determination of real samples.