Screening of carbohydrates in urine by capillary electrophoresis

Abstract

Carbohydrate analysis of urine is clinically important in assisting diagnosis of disorders of carbohydrate metabolism and understanding its pathologic significance. Paper chromatography and thin-layer chromatography are the techniques that are often employed for the determination of urinary carbohydrates. An aim of our experiments was to investigate the utility of capillary electrophoresis to develop a fast screening procedure of urinary carbohydrates. Simultaneous resolution of eight carbohydrates involving maltose, lactose, D-mannose, D-glucose, D-ribose, D-xylose, L-arabinose and D-galactose as markers was obtained with 130 mM borate (pH 10.2). Ethanol/water (80/20 v/v) and acetonitrile proved to be efficient reagents for urine sample clean-up which produced symmetrical peaks. The urine sample from a normal subject was determined to contain lactose, glucose, xylose and arabinose that fall within normal ranges of these carbohydrates in urine. The investigations made in this study may be potentially useful in carbohydrate screening, especially in neonatal urine screening.