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|Title:||Protein microarrays on ITO surfaces by a direct covalent attachment scheme|
|Citation:||Ng, H.T., Fang, A., Huang, L., Li, S.F.Y. (2002-08-06). Protein microarrays on ITO surfaces by a direct covalent attachment scheme. Langmuir 18 (16) : 6324-6329. ScholarBank@NUS Repository. https://doi.org/10.1021/la0255828|
|Abstract:||We describe the use of an indium tin oxide (ITO) thin film on a solid support serving as an effective generic immobilization platform to achieve one-step direct covalent attachment of arrayed proteins with good reproducibility and uniformity, Potential functional analyses on these surfaces involving protein-protein and protein-ligand interactions have been demonstrated. We also show that the approach can be adapted, via a photolithography-derived microwell route, to produce high-density protein microarrays which typically could accommodate a significantly higher density while occupying a comparatively smaller footprint than that achievable using currently existing high-precision robotic microarrayer systems, suggesting its potential applications in simultaneous parallel biological assays.|
|Appears in Collections:||Staff Publications|
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