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Title: ImFCS: A software for imaging FCS data analysis and visualization
Authors: Sankaran, J.
Shi, X. 
Ho, L.Y.
Stelzer, E.H.K.
Wohland, T. 
Issue Date: 6-Dec-2010
Citation: Sankaran, J., Shi, X., Ho, L.Y., Stelzer, E.H.K., Wohland, T. (2010-12-06). ImFCS: A software for imaging FCS data analysis and visualization. Optics Express 18 (25) : 25468-25481. ScholarBank@NUS Repository.
Abstract: The multiplexing of fluorescence correlation spectroscopy (FCS), especially in imaging FCS using fast, sensitive array detectors, requires the handling of large amounts of data. One can easily collect in excess of 100,000 FCS curves a day, too many to be treated manually. Therefore, ImFCS, an open-source software which relies on standard image files was developed and provides a wide range of options for the calculation of spatial and temporal auto- and cross-correlations, as well as differences in Cross-Correlation Functions (ΔCCF). ImFCS permits fitting of standard models to correlation functions and provides optimized histograms of fitted parameters. Applications include the measurement of diffusion and flow with Imaging Total Internal Reflection FCS (ITIR-FCS) and Single Plane Illumination Microscopy FCS (SPIM-FCS) in biologically relevant samples. As a compromise between ITIR-FCS and SPIM-FCS, we extend the applications to Imaging Variable Angle-FCS (IVA-FCS) where sub-critical oblique illumination provides sample sectioning close to the cover slide. © 2007 Optical Society of America.
Source Title: Optics Express
ISSN: 10944087
DOI: 10.1364/OE.18.025468
Appears in Collections:Staff Publications

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