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Title: Formation of Volatile Sulfur-Containing Compounds by Saccharomyces cerevisiae in Soymilk Supplemented with L-methionine
Authors: Quek, J.M.B.
Seow, Y.-X.
Ong, P.K.C.
Liu, S.-Q. 
Keywords: methional
Saccharomyces cerevisiae
Issue Date: Oct-2011
Source: Quek, J.M.B., Seow, Y.-X., Ong, P.K.C., Liu, S.-Q. (2011-10). Formation of Volatile Sulfur-Containing Compounds by Saccharomyces cerevisiae in Soymilk Supplemented with L-methionine. Food Biotechnology 25 (4) : 292-304. ScholarBank@NUS Repository.
Abstract: The formation of volatile sulfur-containing flavor compounds from L-methionine catabolism by Saccharomyces cerevisiae EC-1118 was investigated in soymilk supplemented with this sulfur-containing amino acid with a focus on methionol (3-methylthio-1-propanol). Methionol produced from L-methionine metabolism of yeast in soymilk was extracted by solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). The effects of time (0 - 72 h), L-methionine concentration (0.05-0.20%), yeast extract concentration (0.00-0.30%), initial medium pH (4.5-6.9), and temperature (25-34°C) on the production of methionol were investigated. One-liter scale-up fermentation of soymilk by Saccharomyces cerevisiae EC-1118 was conducted for 48 h under static conditions with 0.15% of L-methionine, 0.10% of yeast extract, initial medium pH of 5.5, and temperature of 25°C, yielding 190 ppm of methionol. GC-olfactometry dilution analysis was conducted on extracts of the fermented soymilk, and no single compound was found to account for the overall odor perception. Methional (3-methylthio-1-propanal) and methionol were determined to be most potent odor-active compounds in the fermented soymilk. The fermented soy milk may serve as a complex sulfur flavor concentrate for applications in foods such as fermented soy products, enzyme-modified, and imitation cheeses. © 2011 Taylor and Francis Group, LLC.
Source Title: Food Biotechnology
ISSN: 08905436
DOI: 10.1080/08905436.2011.617254
Appears in Collections:Staff Publications

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