Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2010.11.095
Title: Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai, S.
Yoon, S.-I.
Murata, A.
Takabayashi, M.
Wu, X. 
Lu, Y. 
Takeoka, S.
Ozaki, M.
Keywords: Fluorescent imaging
His-tag
Small chemical probe
Issue Date: 7-Jan-2011
Source: Arai, S., Yoon, S.-I., Murata, A., Takabayashi, M., Wu, X., Lu, Y., Takeoka, S., Ozaki, M. (2011-01-07). Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells. Biochemical and Biophysical Research Communications 404 (1) : 211-216. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2010.11.095
Abstract: A specific protein fluorescent labeling method has been used as a tool for bio-imaging in living cells. We developed a novel system of switching " fluorescent turn on" by the recognition of a fluorescent probe to a hexahistidine-tagged (His-tag) protein. The tetramethyl rhodamine bearing three nitrilotriacetic acids, which was used as a fluorescent probe to target a His-tagged protein, formed a reversible complex with the quencher, (Dabcyl)-conjugated oligohistidines, in the homogeneous solution, causing fluorescence of the fluorophore to be quenched. The complex when applied to living cells (COS-7) expressing His-tagged proteins on the cell surface caused the quencher-conjugated oligohistidines to be dissociated from the complex by specific binding of the fluorescent probe to the tagged protein, resulting in the fluorescent emission. The complex that did not participate in the binding event remained in the quenched state to maintain a low level of background fluorescence. © 2010 Elsevier Inc.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/76193
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2010.11.095
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