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https://doi.org/10.1016/j.biomaterials.2010.07.088
| Title: | Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147 | Authors: | Eisele, K. Gropeanu, R.A. Zehendner, C.M. Rouhanipour, A. Ramanathan, A. Mihov, G. Koynov, K. Kuhlmann, C.R.W. Vasudevan, S.G. Luhmann, H.J. Weil, T. |
Keywords: | Cationized serum albumin CBSA-147 Isothermal titration calorimetry Polyelectrolyte complex formation Protein transfection agent |
Issue Date: | Nov-2010 | Citation: | Eisele, K., Gropeanu, R.A., Zehendner, C.M., Rouhanipour, A., Ramanathan, A., Mihov, G., Koynov, K., Kuhlmann, C.R.W., Vasudevan, S.G., Luhmann, H.J., Weil, T. (2010-11). Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147. Biomaterials 31 (33) : 8789-8801. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.07.088 | Abstract: | We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability. © 2010 Elsevier Ltd. | Source Title: | Biomaterials | URI: | http://scholarbank.nus.edu.sg/handle/10635/76178 | ISSN: | 01429612 | DOI: | 10.1016/j.biomaterials.2010.07.088 |
| Appears in Collections: | Staff Publications |
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