Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.07.088
Title: Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147
Authors: Eisele, K.
Gropeanu, R.A.
Zehendner, C.M.
Rouhanipour, A.
Ramanathan, A.
Mihov, G.
Koynov, K.
Kuhlmann, C.R.W.
Vasudevan, S.G. 
Luhmann, H.J.
Weil, T. 
Keywords: Cationized serum albumin
CBSA-147
Isothermal titration calorimetry
Polyelectrolyte complex formation
Protein transfection agent
Issue Date: Nov-2010
Citation: Eisele, K., Gropeanu, R.A., Zehendner, C.M., Rouhanipour, A., Ramanathan, A., Mihov, G., Koynov, K., Kuhlmann, C.R.W., Vasudevan, S.G., Luhmann, H.J., Weil, T. (2010-11). Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147. Biomaterials 31 (33) : 8789-8801. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.07.088
Abstract: We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability. © 2010 Elsevier Ltd.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/76178
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2010.07.088
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