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|Title:||Clinical evaluation of amperometric enzyme electrodes in continuous-flow analysis for glucose in undiluted whole blood|
|Authors:||Gunasingham, H. |
|Keywords:||carbon-paste enzyme electrode|
|Source:||Gunasingham, H.,Tan, C.-H.,Aw, T.-C. (1990). Clinical evaluation of amperometric enzyme electrodes in continuous-flow analysis for glucose in undiluted whole blood. Clinical Chemistry 36 (9) : 1657-1661. ScholarBank@NUS Repository.|
|Abstract:||We have evaluated the carbon-paste enzyme/wall-jet electrode in continuous-flow analysis for glucose in undiluted whole blood. Responses of the electrode to protein-based and aqueous samples under wall-jet configuration are described. Imprecision in the flow-injection mode was <2% for glucose values in the usual analytical range (1-25 mmol/L). The dynamic range for glucose was from 0.03 to 45 mmol/L, with about 100% recovery of added substrate. The performance of the electrode (y) in flow-injection and steady-state modes was compared with a colorimetric method (x; Kodak Ektachem XR700), yielding linear-regression equations of y = 1.152x + 0.295 and y = 0.979x + 0.500, respectively. Among the anticoagulants, blood preservatives, and interfering substances investigated, only ascorbic acid gave a significant positive bias. The electrode exhibited good stability for analyses of undiluted whole-blood samples.|
|Source Title:||Clinical Chemistry|
|Appears in Collections:||Staff Publications|
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