Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/75763
Title: Clinical evaluation of amperometric enzyme electrodes in continuous-flow analysis for glucose in undiluted whole blood
Authors: Gunasingham, H. 
Tan, C.-H.
Aw, T.-C.
Keywords: carbon-paste enzyme electrode
glucose oxidase
wall-jet electrode
Issue Date: 1990
Source: Gunasingham, H.,Tan, C.-H.,Aw, T.-C. (1990). Clinical evaluation of amperometric enzyme electrodes in continuous-flow analysis for glucose in undiluted whole blood. Clinical Chemistry 36 (9) : 1657-1661. ScholarBank@NUS Repository.
Abstract: We have evaluated the carbon-paste enzyme/wall-jet electrode in continuous-flow analysis for glucose in undiluted whole blood. Responses of the electrode to protein-based and aqueous samples under wall-jet configuration are described. Imprecision in the flow-injection mode was <2% for glucose values in the usual analytical range (1-25 mmol/L). The dynamic range for glucose was from 0.03 to 45 mmol/L, with about 100% recovery of added substrate. The performance of the electrode (y) in flow-injection and steady-state modes was compared with a colorimetric method (x; Kodak Ektachem XR700), yielding linear-regression equations of y = 1.152x + 0.295 and y = 0.979x + 0.500, respectively. Among the anticoagulants, blood preservatives, and interfering substances investigated, only ascorbic acid gave a significant positive bias. The electrode exhibited good stability for analyses of undiluted whole-blood samples.
Source Title: Clinical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/75763
ISSN: 00099147
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

26
checked on Jan 19, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.