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|Title:||Bitter melon (Momordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells|
|Authors:||Popovich, D.G. |
|Citation:||Popovich, D.G., Li, L., Zhang, W. (2010-06). Bitter melon (Momordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells. Food and Chemical Toxicology 48 (6) : 1619-1626. ScholarBank@NUS Repository. https://doi.org/10.1016/j.fct.2010.03.035|
|Abstract:||Bitter melon (Momordica charantia) contains a variety of potentially bioactive compounds which includes two classes of saponins known as cucurbitane and oleanane-type triterpenoids. A bitter melon (BM) extract was investigated for the potential to reduce cell viability, reduce lipid accumulation in differentiating 3T3-L1 cells and affect subsequent adiponectin expression. BM extract contained at least five different triterpenoids and reduced preadipocyte viability with an LC50 concentration after 24. h determined to be 0.402 ± 0.04. mg/mL, 0.314 ± 0.01. mg/mL for 48. h and 0.310 ± 0.01. mg/mL for 72. h. BM treatment increased the release of lactate dehydrogenase (LDH) from cells and significantly (p<0.05) increased cells in the G2/M while reducing cells in the G1 phase of the cell cycle. BM treatment of 3T3-L1 cells resulted in a decreased in lipid accumulation and significantly (p<0.05) decreased intracellular triglyceride amount compared to untreated control cells. PPARγ expression was significantly (p<0.05) down-regulated. Adiponectin expression was significantly (p<0.05) decreased after 12 and 24. h of treatment and was increased in response to troglitazone, a positive control. BM extract reduced preadipocyte proliferation by a G2/M arrest of the cell cycle and reduced lipid accumulation of the adipocyte. © 2010 Elsevier Ltd.|
|Source Title:||Food and Chemical Toxicology|
|Appears in Collections:||Staff Publications|
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