Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.fct.2010.03.035
Title: Bitter melon (Momordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells
Authors: Popovich, D.G. 
Li, L.
Zhang, W.
Keywords: 3T3-L1
Adipogenesis
Bitter melon
Momordica charantia
PPARγ
Saponins
Issue Date: Jun-2010
Citation: Popovich, D.G., Li, L., Zhang, W. (2010-06). Bitter melon (Momordica charantia) triterpenoid extract reduces preadipocyte viability, lipid accumulation and adiponectin expression in 3T3-L1 cells. Food and Chemical Toxicology 48 (6) : 1619-1626. ScholarBank@NUS Repository. https://doi.org/10.1016/j.fct.2010.03.035
Abstract: Bitter melon (Momordica charantia) contains a variety of potentially bioactive compounds which includes two classes of saponins known as cucurbitane and oleanane-type triterpenoids. A bitter melon (BM) extract was investigated for the potential to reduce cell viability, reduce lipid accumulation in differentiating 3T3-L1 cells and affect subsequent adiponectin expression. BM extract contained at least five different triterpenoids and reduced preadipocyte viability with an LC50 concentration after 24. h determined to be 0.402 ± 0.04. mg/mL, 0.314 ± 0.01. mg/mL for 48. h and 0.310 ± 0.01. mg/mL for 72. h. BM treatment increased the release of lactate dehydrogenase (LDH) from cells and significantly (p<0.05) increased cells in the G2/M while reducing cells in the G1 phase of the cell cycle. BM treatment of 3T3-L1 cells resulted in a decreased in lipid accumulation and significantly (p<0.05) decreased intracellular triglyceride amount compared to untreated control cells. PPARγ expression was significantly (p<0.05) down-regulated. Adiponectin expression was significantly (p<0.05) decreased after 12 and 24. h of treatment and was increased in response to troglitazone, a positive control. BM extract reduced preadipocyte proliferation by a G2/M arrest of the cell cycle and reduced lipid accumulation of the adipocyte. © 2010 Elsevier Ltd.
Source Title: Food and Chemical Toxicology
URI: http://scholarbank.nus.edu.sg/handle/10635/75677
ISSN: 02786915
DOI: 10.1016/j.fct.2010.03.035
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