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Title: Amelogenin processing by MMP-20 prevents protein occlusion inside calcite crystals
Authors: Bromley, K.M.
Lakshminarayanan, R. 
Thompson, M.
Lokappa, S.B.
Gallon, V.A.
Cho, K.R.
Qiu, S.R.
Moradian-Oldak, J.
Issue Date: 3-Oct-2012
Citation: Bromley, K.M., Lakshminarayanan, R., Thompson, M., Lokappa, S.B., Gallon, V.A., Cho, K.R., Qiu, S.R., Moradian-Oldak, J. (2012-10-03). Amelogenin processing by MMP-20 prevents protein occlusion inside calcite crystals. Crystal Growth and Design 12 (10) : 4897-4905. ScholarBank@NUS Repository.
Abstract: Calcite crystals were grown in the presence of full-length amelogenin and during its proteolysis by recombinant human matrix metalloproteinase 20 (rhMMP-20). Recombinant porcine amelogenin (rP172) altered the shape of calcite crystals by inhibiting the growth of steps on the {104} faces and became occluded inside the crystals. Upon co-addition of rhMMP-20, the majority of the protein was digested resulting in a truncated amelogenin lacking the C-terminal segment. In rP172-rhMMP-20 samples, the occlusion of amelogenin into the calcite crystals was drastically decreased. Truncated amelogenin (rP147) and the 25-residue C-terminal domain produced crystals with regular shape and less occluded organic material. Removal of the C-terminal diminished the affinity of amelogenin to the crystals and therefore prevented occlusion. We hypothesize that hydroxyapatite (HAP) and calcite interact with amelogenin in a similar manner. In the case of each material, full-length amelogenin binds most strongly, truncated amelogenin binds weakly, and the C-terminus alone has the weakest interaction. Regarding enamel crystal growth, the prevention of occlusion into maturing enamel crystals might be a major benefit resulting from the selective cleavage of amelogenin at the C-terminus by MMP-20. Our data have important implications for understanding the hypomineralized enamel phenotype in cases of amelogenesis imperfecta resulting from MMP-20 mutations and will contribute to the design of enamel inspired biomaterials. © 2012 American Chemical Society.
Source Title: Crystal Growth and Design
ISSN: 15287483
DOI: 10.1021/cg300754a
Appears in Collections:Staff Publications

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