Please use this identifier to cite or link to this item: https://doi.org/10.1021/cg300754a
Title: Amelogenin processing by MMP-20 prevents protein occlusion inside calcite crystals
Authors: Bromley, K.M.
Lakshminarayanan, R. 
Thompson, M.
Lokappa, S.B.
Gallon, V.A.
Cho, K.R.
Qiu, S.R.
Moradian-Oldak, J.
Issue Date: 3-Oct-2012
Citation: Bromley, K.M., Lakshminarayanan, R., Thompson, M., Lokappa, S.B., Gallon, V.A., Cho, K.R., Qiu, S.R., Moradian-Oldak, J. (2012-10-03). Amelogenin processing by MMP-20 prevents protein occlusion inside calcite crystals. Crystal Growth and Design 12 (10) : 4897-4905. ScholarBank@NUS Repository. https://doi.org/10.1021/cg300754a
Abstract: Calcite crystals were grown in the presence of full-length amelogenin and during its proteolysis by recombinant human matrix metalloproteinase 20 (rhMMP-20). Recombinant porcine amelogenin (rP172) altered the shape of calcite crystals by inhibiting the growth of steps on the {104} faces and became occluded inside the crystals. Upon co-addition of rhMMP-20, the majority of the protein was digested resulting in a truncated amelogenin lacking the C-terminal segment. In rP172-rhMMP-20 samples, the occlusion of amelogenin into the calcite crystals was drastically decreased. Truncated amelogenin (rP147) and the 25-residue C-terminal domain produced crystals with regular shape and less occluded organic material. Removal of the C-terminal diminished the affinity of amelogenin to the crystals and therefore prevented occlusion. We hypothesize that hydroxyapatite (HAP) and calcite interact with amelogenin in a similar manner. In the case of each material, full-length amelogenin binds most strongly, truncated amelogenin binds weakly, and the C-terminus alone has the weakest interaction. Regarding enamel crystal growth, the prevention of occlusion into maturing enamel crystals might be a major benefit resulting from the selective cleavage of amelogenin at the C-terminus by MMP-20. Our data have important implications for understanding the hypomineralized enamel phenotype in cases of amelogenesis imperfecta resulting from MMP-20 mutations and will contribute to the design of enamel inspired biomaterials. © 2012 American Chemical Society.
Source Title: Crystal Growth and Design
URI: http://scholarbank.nus.edu.sg/handle/10635/75547
ISSN: 15287483
DOI: 10.1021/cg300754a
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

6
checked on Oct 16, 2018

WEB OF SCIENCETM
Citations

6
checked on Oct 16, 2018

Page view(s)

25
checked on Oct 5, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.